James Ferguson

Results 228 comments of James Ferguson

Hello, This is probably because you don't have tkinter installed. You can either try installing that, or try these 2 solutions. 1. comment out line 3 in segmenter.py `#matplotlib.use('TkAgg')` 2....

Hello, Yes segmenter would probably be the tool to use. Estimation could be done roughly from sequencing speed and sampling rate, although it would be pretty rough estimate. James

Hey, Ahh yes something like that. Let me look at that and get back to you. Need to have a look at it, it's been a few years... Also are...

Hey, do you have an example read you could share with me that has one of these homopolymers in it? It would help me a lot with what the expected...

That would also solve the issue I just posted about requiring randomised fastq files.

Hey, I'm building some 2x 4090 builds at the moment. When I'm done I'll post some benchmarks ☺️ James

I've built a system with 2x4090 cards. Running software like dorado, Dorado server or my software wrapper buttery-eel will go quite well. 100% utilisation and it flies along at ~1M...

Hey George, As always, very helpful. For all the fields, are they calculated by MinKNOW and provided to the pod5 writer **as is**, or is there some extra calculation that...

Yep, that helps. That's all for now. I'll be sure to ping again if I run into issues. As for now, I've got a working s2p and p2s converter for...

Hey, sorry just re-opening this for a quick related question. ``` [fields.num_minknow_events] type = "int8" description = "Number of minknow events that the read contains" ``` The spec shows this...