James Ferguson
James Ferguson
Currently, only the highest scoring hit. I plan on allowing it to record more than the the top hit. A round about way of "hacking" a solution, is to just...
Hello, Ahh, yes. I need to push the latest update with multi fast5 support for that. I'm at a conference the next 2 days, I'll try and find the time...
Mutli-fast5 support will be released in the next few weeks, however if you use SquigglePull on the multi-fast5 file with the multi-fast5 command on `--multi` , then use MotifSeq on...
Hey, So you want the base positions of what you have found in the signal? Or the signal positions of what you found in the basecall? If either of those...
Hey, That actually sounds rad. Wanna send me an email at j.ferguson[at]garvan.org.au ? I think this would be worth including in the development to ensure we deliver in a way...
Hey, Yep, i got it. I have been talking with the relevant people. Looks like we will be going ahead. I'll be in touch soon.
Hello Quentin, I'll have a look into this. Is the goal here to split your fast5 files into your barcode groups? James
Ahh i think i found the problem. So first, they changed the header in the sequencing summary file from `filename_fast5 read_id to `filename read_id` So the column detection code doesn't...
Okay, if you can do a `git pull` in the repo, and try again? Potential fix made https://github.com/Psy-Fer/SquiggleKit/commit/adbc52e5f1fa06b9b7c7f4a9b71f48c9fba132bb James
Also, I should probably mention, that another way to do this is to convert the files to slow5 using [slow5tools,](https://github.com/hasindu2008/slow5tools) and then it is quite simple to extract the readIDs...