James Ferguson comments

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                                            James Ferguson

SquiggleKit can be used for extract raw current signal in specific position of read?

Hello, Yes, I've thought of the same functionality, and it wasn't quite in the scope of squigglekit at the time of building it. However, I have since built a library...

output files made by SquigglePull.py containing nothing

Hello Ping, Sure. Could you please tell me a few things? Python version? Single or multi fast5 files? Command being used? Operating system? Thanks!

output files made by SquigglePull.py containing nothing

Thanks for that Ping. Try chaning the command to just the top folder, rather than the individual file `python SquigglePull.py -r -p downloads/SquiggleKit/example/ > data6.tsv` The `-p` is meant to...

output files made by SquigglePull.py containing nothing

Ahh, try adding the `--multi` flag

output files made by SquigglePull.py containing nothing

Ahh. That is weird. Let me do some tests to se if I can figure it out.

Fast5_fetcher_multi.py no output

Hey, Yea a bit of work needs to be done since they modified and have multiple versions of, the summary files now. I can do a quick edit for this...

Issue visualizing squiggles with recent fast5 file

Hey, welcome to github. So the error in squigglePlot seems to a missing argument, for the specific readID you want to plot in the fast5 file. Try copying the fast5...

Issue visualizing squiggles with recent fast5 file

Hello, Could you please give me the command you are using? At a quick glance this looks like --save was used without a valid file or --save_path path. Cheers, James

Using RNA for MotifSeq

Hello, yes, just use the following flag `--scrappie_model squiggle_r94_rna`

Using RNA for MotifSeq

Could you please give me an example of the output? I'll look into it. I have some RNA data on hand to double check.