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Multi-subject Single Cell Deconvolution

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Hi, I'm sorry if this is not the place but I cannot find any other resources. The MuSiC deconvolution tutorial is in 2 parts. One is where they deconvolve pancreatic...

Hi, Thank you for such a well explained tutorial. You may want to change the MuSiC-output-list name in the following lines of your tutorial: m.prop.GSE50244 = rbind(melt(**GSE50244.EMTAB.prop**$Est.prop.weighted), melt(**GSE50244.EMTAB.prop**$Est.prop.allgene)) GSE50244.EMTAB.prop -->...

Hello Xuran, Thank you very much for creating this package, we have found it helpful and are still trying to figure out some issues. I have been trying to follow...

Dear Xuran, I first tried to install the package in the suggested way without success: > devtools::install_github('xuranw/MuSiC') Error in read.dcf(path) : Found continuation line starting ' plyr, ...' at begin...

Hi Xuran, I tried to apply MuSiC to RNAseq bulk data with RPKM as the input. According to your paper(Discussion), "MuSiC can utilize RPKM if estimates of cell type-specific total...

Hi, I have bulk RNA-seq from islets cell populations (alpha, beta, delta cells etc.), and am interested in analyzing the content of each sample. For example, I would like to...

Is it possible to specifically turn off the gene length bias correction step when running MuSiC? - I want to try applying MuSiC to nanostring gene expression data. But since...

Hello what does the below mean, how do i fix it. Creating Relative Abudance Matrix... Creating Variance Matrix... Creating Library Size Matrix... Used 17983 common genes... Error in music_prop(bulk.eset =...

Hi, I am trying to run the code below and get an error. Help would be appreciated. Code: library(BisqueRNA) library(xbioc) library(MuSiC) library(Biobase) gse132771_all_eset

Would it be possible to share the R code for computing the true cell type proportions in the artificial bulk data in your tutorial? That will help me (and I...