Richard Harris

Thanks for the update - we'll take a look at this and get back to you.

@cocvincent - Can you share some information on your system OS etc?

Could you tell me your glibc version?

Ok - this reminded me of this similar issue https://github.com/nanoporetech/pod5-file-format/issues/63 The glibc version you have is too old: https://github.com/nanoporetech/pod5-file-format/issues/63#issuecomment-1655312893 Are you able to compile the project yourself? https://pod5-file-format.readthedocs.io/en/latest/docs/install.html#installation-from-source

Hi @peradastra, The error is telling you what to do as is the following note in the [documentation](https://pod5-file-format.readthedocs.io/en/latest/docs/tools.html#pod5-filter) > [!NOTE] >The filter and subset tool will assert that any requested...

Hi @Macdot3, Can you share the full command that you run please? Kind regards, Rich

From your command I don't see any issues or any reason why it would need to access `/tmp` as any temp files created by pod5 are written locally. Could you...

POD5_DEBUG will have generated `.log` files in the working directory - could you share those please?

This error can occur from HDF5 not finding the plugin to open the fast5 files. Can you please ensure that you have `vbz_h5py_plugin` installed in the python environment? Kind regards,...

@Macdot3, The log files show that this could be a problem with HDF5 ``` File "/home/barresi.m/Nanopore/Dorado/POD5_ENV/lib/python3.12/site-packages/pod5/tools/pod5_convert_from_fast5.py", line 540, in convert_fast5_read signal = raw["Signal"][()] ~~~~~~~~~~~~~^^^^ ``` As for the plugin: ```...