Richard Harris
Richard Harris
You might be missing a whitespace `-k 14`
Can you add verbose logging with `-vv` please?
Yes - It looks like you're running out of memory. --- Also this **isn't the same command** you were having issues with as it doesn't contain the `--modified-bases` argument at...
@JhinJhinJhin, there are no options to control the number of CPUs used by dorado - it will use as many as there are available. Please use a GPU to get...
@Taylorain, > 1. When using the [email protected]_inosine_m6A@v1 model, I observed that both inosine (I) and m6A modifications were detected at the same site. There will be predictions for both inosine...
Hi @priyanagpal25, > for an individual bam file: samtools view -H fastq_runid_e77a6fda925a5796b8b74964b42548a9fe2be7ec_6_1.bam | grep -E "MM:|ML:" The issue here is that you have the `samtools view -H` flag set so...
@skranz0, From the list of [supported modification](https://dorado-docs.readthedocs.io/en/latest/basecaller/mods/#supported-modifications) we see that 6mA and m6A are modifications supported by dorado but exclusive to DNA and RNA respectively, so this subtlety does matter....
Hi @DntBScrdDv ~~This is intended behaviour as stated in the `--help`:~~ ```text -resume-from Resume basecalling from the given HTS file. Fully written read records are not processed again. ``` ~~You'll...
Hi @DntBScrdDv, I've had a check and you're correct my apologies - dorado should be copying the reads into the new output [src](https://github.com/nanoporetech/dorado/blob/release-v0.7/dorado/read_pipeline/ResumeLoaderNode.cpp#L59). We'll clarify that in the --help documentation...
I did some testing locally to see what effect various levels of compression would have on the output and there's approx 2x reduction from min and max compression. But nowhere...