Richard Harris

Can you try again and add ```pip3 --version``` after the upgrade? Even though it says `Successfully installed pip-24.0` - `Not uninstalling pip at /usr/lib/python3/dist-packages, outside environment /usr` is a bit...

Hi @mp15, Would you be able to add `POD5_DEBUG=1` next time to hopefully capture in more detail what's going wrong here? The number of "threads" ([which are actually processes](https://pod5-file-format.readthedocs.io/en/latest/docs/tools.html#tools)) `-t...

Hi @DCossey , Yes it is possible to fix your metadata although it's not particularly clean as pod5 files are immutable. There is a short part of the [documentation](https://pod5-file-format.readthedocs.io/en/latest/docs/api.html#adding-reads-example) relating...

What command are you running?

Can you try without the quotes please?

Can you run the following then: ```python pod5 --version POD5_DEBUG=1 pod5 view output2.pod5 ``` And then share the contents of the pod5 `.log` files that are generated?

ah - this could be an new issue from `polars==0.20` Can you please ensure you're using `polars==0.19` If not please re-install `polars` with `pip install -U polars~=0.19`

Fantastic, sorry about that last issue - we're patching this as we speak

Hi @olawa , The convert tool has a the `--one-to-one` option mentioned in the [documentation](https://pod5-file-format.readthedocs.io/en/latest/docs/tools.html#pod5-convert-fast5) where a pod5 file is created per fast5 if this is the file arrangement you...

Hi @sklages, unfortunately not - I've had to pause that work for a little while. Hopefully I'll have time to back to it soon and I'll let you know when...