nanopolish
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jts
Signal-level algorithms for MinION data
Hi, Whenever i run a nanopolish polya run with my dRNA dataset i get an empty file with each read saying 'NO FILE LOADED'. Commands and output as below: Command:...
Hi,I'm running the module how to estimate poly(A) tail lengths from nanopore native RNA reads, and I've got the result file, but I don't understand the result file right now,...
Hi, I'm currently trying to install nanopolish on a linux server and have used the following: $git clone --recursive https://github.com/jts/nanopolish.git cd nanopolish make After running for a few minutes, the...
Installation fails. Can’t access the HDF5 site. Any plans to fix this again or is nanopolish not accessible to those that didn’t install 2 years ago?
I have a run with multiple barcoded samples that I have demultiplexed and am attempting to run nanopolish polya on. I am using both the "passed" fast5 files and the...
Hi, I'm trying to use nanopolish to generate consensus sequences from bam files. I am finding that 'nanopolish variants' is using very few of the reads located by 'nanopolish index'....
Hello, I managed to run through the example provided so I know the code is working. But when I used a bit of sample data I generated a while back...
Hi, My data is pod5 data.And I converted the pod5 data to fast5 data and analyzed it using nanopolish，but there is no result in naopolish result。Here is my code and...
Hi, I am analyzing m6A call from nanopore direct RNA sequenceing data. After basecalling, the direct RNA reads contain U instead of T. I wonder if it is necessary to...
Hello, I'm using nanopolish call-methylation on an Ontario long read data with an average read depth of 35. I'm using 40 processors with 4 GB of memory per processor. I'm...
