Do I need to convert U to T for nanopore direct RNA m6A analysis

[wuy24]

Hi,

I am analyzing m6A call from nanopore direct RNA sequenceing data. After basecalling, the direct RNA reads contain U instead of T. I wonder if it is necessary to conver U to T in fastq file in order to do m6A calling. And if I converted U to T in the analysis, will it affect the results? Thank you!

Best, Ying