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nanopolish polya outputting NO FILE LOADED
Hi, Whenever i run a nanopolish polya run with my dRNA dataset i get an empty file with each read saying 'NO FILE LOADED'. Commands and output as below:
Command: nanopolish index -d /20230927_1043_MN38033_FAX31978_99230b36/fast5_pass/ -s ./sequencing_summary.txt ./pass/polya.fastq
Output: [readdb] indexing /mnt/lustre/RDS-20230927_1043_MN38033_FAX31978_99230b36/fast5_pass/ [readdb] num reads: 1252505, num reads with path to fast5: 1252505
Command: minimap2 -a -x map-ont ../OM830318.fasta polya.fastq > polya.sam
Command: nanopolish polya --verbose --reads polya.fastq --bam polya.sorted.bam --genome ../OM830318.fa > polya_results.tsv
Output: [post-run summary] total reads: 601580, unparseable: 0, qc fail: 0, could not calibrate: 0, no alignment: 0, bad fast5: 300790