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pod5 to fast5 and nanopolish call-methylation no reult
Hi,
My data is pod5 data.And I converted the pod5 data to fast5 data and analyzed it using nanopolish,but there is no result in naopolish result。Here is my code and log information。
nanopolish call-methylation -t 4 -r J-365033-ZM-3.all.fastq -b J-365033-ZM-3.merge.Y.bam -g /mnt/data/human_g1k_v37.fasta > methylation_calls.tsv
### [post-run summary] total reads: 52290, unparseable: 0, qc fail: 0, could not calibrate: 0, no alignment: 52290, bad fast5: 171