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Rapid large-scale prokaryote pan genome analysis

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"![Screenshot from 2022-08-01 11-42-35](https://user-images.githubusercontent.com/72471432/182079967-4ad0d767-5528-4c30-bc14-10cbfdf364ea.png) I'm fairly new to this and I'm getting this when i type in " roary -e --mafft -p 8 -f ROARY_dir **.gff what should i do?

Hello all, I have annotated a number of genomes with Prokka which they contain a gene of interest in their annotated files. However after Roary analysis (-i 80 and -cd...

Hi, First of all, love the program! Just have a question though - while running a job on time-restricted resources on a remote server the resource allocation timed out while...

Hello, I am using the program roary_plots.py with a modified matrix inclding only a few genes that I'm interested in. This means that each gene can be clearly seen in...

Hello from Japan. Now I'm analyzing 38 gff files of specific species of bacteria. Here is my code for running roary. `${SINGULARITY} exec --cleanenv ${ROARY_SIF} roary -p ${THREADS} -e --mafft...

Hello, I would like to use Roary to analyze the GFF files of 800 vibrio and 30 non-vibrio. What configuration do I need for the server? Is a server with...

Hi, i am trying to install roary but it gives this error ? could you please help me? roary : Depends: r-cran-ggplot2 but it is not going to be installed...

Hello I have been trying to run roary to build a tree for a whole genus. The program starts fine, it built the 'clustered' and 'combined' files ok. It started...

Hi, I am trying to build the pan genome for ~200 environmental E. coli isolates. MG1655 was included in the data set for reference. I found Roary has a tendency...

when i used roary to construct the pangenome it gave presence_absences.csv file , in that the third column is about protein cluster annotation. and it gives alot of hypothetical proteins...