Robert Vaser
Robert Vaser
Hello, can you please paste the mapping/alignment command? You probably misplace the reads/reference file in the Racon command. Best regards, Robert
Are you trying to polish contigs with reads or with the same (modified?) contigs? If second, there might be two sequences in `purged.fa` and `fp.contigs.fasta` which share the same name...
Can you explain what you are trying to do?
Hello, can you please try the latest version from https://github.com/lbcb-sci/racon? Best regards, Robert
Actually, bioconda has v1.5.0 as well so please update and see if the error persists.
The error unequal lengths indicates that the FASTA/Q files you used in minimap2 and the ones passed to racon have differences (offending read is `ec458864-7c3c-492e-afa8-2a9f7fbe5bef`). Did you by any chance...
Hi, are you using the latest conda version of Racon? Try compiling from source on your machine (the error indicates that your CPU does not support SIMD instructions with which...
Hello Hongbing, if you want all of your contigs in the output file, regardless of them being polished or not, use option `-u`/`--include-unpolished`. You can just use raw reads directly...
Hi, please run `make VERBOSE=1`, check the compiler that is being used and paste the version here. Best regards, Robert
Hi Hequan, unfortunately no. Best regards, Robert