Jared Simpson
Jared Simpson
Sorry, I misread your issue initially (I shouldn't try to answer emails first thing in the morning...). Splitting the fastq would work, but isn't the recommended way since it will...
Hi, Thanks for including the full output, the most relevant lines are here: ``` [post-run summary] total reads: 39192, unparseable: 0, qc fail: 0, could not calibrate: 159, no alignment:...
Try using [NanoPlot](https://github.com/wdecoster/NanoPlot), which will produce many QC plots for you to look over. If you send some I will be happy to help interpret them. If you provide it...
The quality plot looks fine, I wouldn't expect that to cause problems with nanopolish. The other plot is not the one I was looking for, it should be titled something...
That looks fine too, I don't think there is an issue with the data quality. I'm unsure what the problem is. I notice the input file name is `filtered_barcode01.fastq` -...
Do you get any output in the `fail.vcf` files?
Sure, if you can package the fast5 and fastq I can take a look. It is a long weekend here and I have a few other pressing tasks so I...
@OgnjenMilicevic can you paste the guppy commands you ran?
Thanks. `FLO-MIN111` indicates this is an R10 flowcell which nanopolish does not yet support in `master` or the tagged releases. There is an `r10` branch on github that has experimental...
@rekham1077 can you try the `r10` branch?