Jared Simpson

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Glad to hear it built successfully

It works with guppy too.JaredOn Sep 18, 2023, at 11:50 PM, sunsetyerin ***@***.***> wrote: Is it possible to "nanopolish polya" with guppy 6.0.6 called fastq files? Or do only albacore...

It looks like your data is R10, please see here: https://github.com/jts/nanopolish#a-note-on-r10-support

Sorry but we do not support R10 for `nanopolish eventalign` yet - you may wish to look at f5c as it does support R10 data (cc: @hasindu2008)

Hi, Are you using the latest nanopolish? The final log message suggests you're using an older version as there was a double-counting bug in some old versions. Jared

The error message (`__cray_sset_detect`) suggests you are using a Cray computer. What architecture is the machine? Can you post the output of `uname -a`?

Thanks. It looks like your development environment is based on Cray, which differs from the gnu dev environment that nanopolish expects. You should contact your sysadmins for help on compiling...

Hi @lopp1Y, Before running `nanopolish polya` you should have run `nanopolish index`, which builds a map from the basecalled reads (fastq) to the signal reads (fast5/pod5). This allows nanopolish to...

The person who was responsible for building the read the docs site is no longer in my group and I haven't found the time to do it myself - I'll...

> I was not able to use "nanopolish index" to index the fast5 file because I unfortunately do not have access to the fast5 file. I don't know how much...