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Tools to process and analyze deep sequencing data.

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Deeptools 3.5.1 ``` plotCoverage -b SRR105321441.dedup.bam -p 4 --outRawCounts coverage.tab --plotFile sample_coverage --plotTitle "Sample Coverage" --verbose -o sample_coverage.png ``` ``` File "countReadsPerBin.py", line 713, in get_coverage_of_region multiprocessing.current_process().name, c, c /...

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Hi everyone, I use bamCoverage (bamCoverage version: 2.5.3, python version: 2.7.5) to convert sorted bam files to bigwig files for visualization in UCSC genome browser. It usually takes less than...

Hello! I have a question about making bigWig files using bamCoverage. When I make bigWig files, all the samples have different mapped reads. And also, there are many different normalization...

Hi, thanks for implementing the new function 'dataRange'. I find it very useful. However, I stumbled across an issue and have one feature request: # The issue nan values in...

enhancement

Hello, I have chip-seq data for several histone marks for which I have created RPKM normalized bigwig tracks. I am now looking at the correlation between them using multiBigwigSummary and...

Hello, Thank you for giving nice program. I have a question about sorting heatmap. I'm using '--sortUsing' option when I draw heatmap. In manual, there is no detailed explanation about...

Current behavior is to produce a plot with the number of samples equal to the number of labels provided, presumably matched by index such that samples may be labelled incorrectly...

Hi, I think there is a small bug in the plotProfile output (v3.2.1) and MultiQC. ``` computeMatrix reference-point \\ --referencePoint TSS \\ -R $geneBed \\ -S ${bigwig} \\ -o ${prefix}_matrix.mat.gz...

Hi, I met problems when I run the computeMatrix program. The errors are as following: File "miniconda/computeMatrix", line 14, in main(args) File "/miniconda/lib/python3.7/site-packages/deeptools/computeMatrix.py", line 421, in main hm.computeMatrix(scores_file_list, args.regionsFileName, parameters,...