hifiasm
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chhylp123
Hifiasm: a haplotype-resolved assembler for accurate Hifi reads
I am working on an assembly for an expected genome size of 2.65Gbp. We have HiC data, and 6 SMRTCells worth of HIFI data (3 from a male, and 3...
Dear prof. Heng Li, Thank you so much for the tool! I have been using hifiasm since 2021, and am a big fan of it. I recently run into different...
Hi, We recently recieved the complete genome sequence of a bacterium that contains one chromosome and two plasmids assembled with HiFiasm. The chromosome is assembled normaly and it is labeled...
Hello, I have a diploid plant of ~ 1000 MB haploid size, and we have ~65x hifi coverage of the haploid genome, meaning >30x per haplotype. The quality of the...
Hello, I was wondering whether there are any plans to incorporate support for Pore-C data instead of HiC for phasing? I assume the multiway contacts and longer fragment lengths would...
Hi I can't find a documentation to rerun from ec.bin file with new parameter and new output name could you give me an example how to rerun from ec.bin files....
Can you explain how b filter is working what is the impact of b0 b15 and b37 on kmer number and accuracy for human
Hi @chhylp123 I have 2 assemblies, one with only HiFi data (50x) and HiFi (60x) + OmniC (30x). I was expecting with addition of OmniC, hamming error should improve compared...
Hi, I have HiFi+ONT+HiC data for several samples carrying chromosomal translocations. For a few samples `hifiasm` does not catch the translocations, cases that `verkko` is able to resolve. I tried:...
Hi! I am running Hifiasm v0.19.5-r593 with Hifi and ultra long oxford nanopore reads. However, after a few days running the process fail (core dump). I performed a selection of...
