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Genome annotation with AUGUSTUS

Results 127 Augustus issues
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Hello, This is not an issue but rather a question. I am using Augustus v2.7. After reading the manual I gave Augustus a repeat softmasked file, expecting the command to...

I wondered if there were any update planned to support **ab intio** prediction of splice sites outside of gt-ag and gc-ag. I'm aware there is support for annotating non-canonical sites...

A user informed us that WebAUGUSTUS currently rejects his hints file wiht source=M hints. The file looks like this: ``` CTG93 EVMpout start 124803 124805 0 - 0 source=M CTG93...

It does not seem one needs any of the following to link: `$(HTSLIB)/libhts.a -lcurses -lm -lz -lpthread -lcurl -lssl -lcrypto`, only `-lbam` is needed.

Hi. How can I configure Augustus to seek the Boost libs in a different location? ``` cd utrrnaseq/Debug; make all; make[2]: Entrando no diretório `/usr/local/augustus/3.3.2/gcc-9.1.0/auxprogs/utrrnaseq/Debug' Building file: ../src/Compute_UTRs.cpp Invoking: GCC...

Hello! I have been struggling for a while to get bam2hints to compile: `make[2]: Entering directory `/netdata/maxcoyle/software/Augustus/auxprogs/bam2hints' g++ -Wall -O2 -Wall -O2 -c bam2hints.cc -o bam2hints.o /netdata/maxcoyle/software/bamtools-master/usr/local/include bam2hints.cc:16:27: fatal error:...

bamtools not where the default build expects. Had to change this Makefile as shown below. Note in particular the addition of "/include" in the INCLUDES line. ``` diff auxprogs/filterBam/src/Makefile auxprogs/filterBam/src/Makefile.dist...

The pre-compiled augustus (http://bioinf.uni-greifswald.de/augustus/binaries/augustus-3.4.0.tar.gz) raises error: ``` ./augustus: /lib64/libm.so.6: version `GLIBC_2.29' not found (required by ./augustus) ./augustus: /lib64/libc.so.6: version `GLIBC_2.14' not found (required by ./augustus) ./augustus: /lib64/libstdc++.so.6: version `GLIBCXX_3.4.26' not...

I am trying to reproduce the "Predicting Genes in Single Genomes with AUGUSTUS" tutorial: https://pubmed.ncbi.nlm.nih.gov/30466165/ When I tried to reproduce the "Filtering BAM" step using the filterBam program, I received...

Hi, I have had problems with the next Augustus run. Is the "--singlestrand" option compatible with prokaryotic models? Command line: augustus --species=sulfolobus_solfataricus --strand=both --singlestrand=true --genemodel=complete --protein=off --introns=on --start=on --stop=on --cds=on...

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