16S-rDNA-V3-V4
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16S rDNA V3-V4 amplicon sequencing analysis using dada2, phyloseq, LEfSe, picrust2 and other tools. Demo: https://ycl6.github.io/16S-Demo/
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16S-rDNA-V3-V4 issues
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The current practice is to randomly pick a taxa (with `sample`) and assign it as outgroup. There have been suggestions to do this differently. Need to re-visit this when free....
I'm choosing 4.0x version of your project are currently being supported with security updates.