Joyjit Daw
Joyjit Daw
good catch @smaegol ! we are turning off primer and adapter trimming but didn't account for barcodes. Will get that fixed in the next release!
@ArnaudStigliani @MustafaElshani - are you able to redo your runs with `--no-trim` added as well to see if that addresses your issues?
Hi @MustafaElshani - rerunning with 0.5.3 shouldn't be required for your case, and I doubt the conversion of fast5 to pod5 is causing this issue. Would you be open to...
thanks you! I'll have a look at this right away
Hi @MustafaElshani - sorry I forgot to get back to you on this thread! I was able to reproduce what you're seeing and I've been able to find some improvements...
Hi @obegik - thanks for the request! we are working on this feature and hope to have this available as part of the next release (sometime in March)
Hi @obegik - unfortunately that won't work right now since the adapters we look for polyA are hard coded. It should be quite straightforward to edit that and rebuild dorado...
Yes we take into account both ends to determine the orientation of the cDNA and where to expect the tail. If it's only single ended for you, can you post...
gotcha - okay then I would suggest changing the algorithm the following way - In https://github.com/nanoporetech/dorado/blob/release-v0.5.3/dorado/read_pipeline/PolyACalculator.cpp#L214 1. Set VNP to your custom flank sequence 2. comment out line using any...
Hi @obegik we are working on enabling some features to help your use case. would you be open to sharing some sample data so we can test our solution?