Joyjit Daw comments

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                                            Joyjit Daw

Dorado Tests Failed After Successful Compile

Filling in the template I posted will help answer your questions :) . e.g. how large is your dataset for duplex? were you running without or without alignment (i.e. what's...

Discrepancy between MinKNOW and dorado

Hi @tnn111 - as @HalfPhoton can you post the cmd you ran and the version of dorado used? Also, was demultiplexing and barcode trimming enabled in MinKNOW? Can you check...

Discrepancy between MinKNOW and dorado

@tnn111 do you have any updates?

Number of classified reads significantly differ between ont-dorado-server and dorado standalone

Hi @billytcl - can you try to run the `dorado basecaller` command with then `--no-trim` option? I suspect that adapter trimming may be getting in the way of barcode classification,...

Number of classified reads significantly differ between ont-dorado-server and dorado standalone

Hi @billytcl - I want to confirm that's indeed the case for your situation. We have some improvements along that line internally for the next release already, and your input...

Number of classified reads significantly differ between ont-dorado-server and dorado standalone

Closing due to inactivity. FYI @billytcl we have improved the interplay between adapter trimming and barcoding within dorado since 0.6.0 release, so this should be less of a problem now

How does chimeric read splitting work and how to take advantage of additional adapters

Hi @pclavell - thanks for raising the issue. > it does not reach 100% sensitivity What kind of sensitivity are you seeing? The DNA read splitting code is [here](https://github.com/nanoporetech/dorado/blob/release-v0.5.3/dorado/splitter/DuplexReadSplitter.cpp). Dorado...