Robert Vaser
Robert Vaser
If you have long reads than yes, you can just sum up the file sizes and add some epsilon (add the target size twice because of the newly generated consensus,...
Timestamps and reduced log is implemented in latest commit (`v1.3.3`).
Hello, if you have enough data, all sequences will be polished. If you run racon with `-u`, the output file will contain both polished and unpolished sequences. Best regards, Robert
The commands look fine, but you might not get optimal results for RNA-sequencing due to alternative splicing. Your best bet is to additionally use option `-f`.
Unfortunately, you cannot get the identifiers of sequences used for polishing of each other sequence. You could map the polished result with itself and see if any two sequences have...
Hello Huandna, can you please paste here output of `head -n 4 reads.fasta`? Best regards, Robert
Aren't you missing `>` before each sequence name?
If the above snippet is the result of the head command, then you are missing `>`.
The raw text has them, my bad :) Can you run `tail -n 4 reads.fasta`?
That looks fine as well, hmm. Can you run the same for `draft.fasta` please? Also, please check the size of `ONTmin_IT0.paf`.