Robert Vaser
Robert Vaser
Hello, what is the purpose of this test you conducted? Do you want to test read error correction or contig polishing? Best regards, Robert
This might occur but there is no guarantee for it. If you have repetitive regions without any overhangs left or right, it is highly likely that there will be multiple...
Hi, unfortunately no. You could try this: https://github.com/isovic/racon/issues/9#issuecomment-275067339 Best regards, Robert
Hi, can you paste the command you are using? Best regards, Robert
Could you please copy the minimap2 command you used to get the .paf file?
Hi, it appears that you misplaced the reference file, i.e. in the minimap2 command you are mapping reads to `98ZLc_assembly_NP.fasta` and in the racon command you want to polish `98ZLc_nanopore_filt_ava_assembly.fasta`....
It appears again that you misplaced the files :D You are mapping `98ZLc_combineraw_IL.fastq` to your assembly with minimap2 and in racon you are using different reads `98ZLc_nanopore_raw.fastq`. These two files...
Was the Illumina file `98ZLc_combineraw_IL.fastq` created by joining 2 paired end files?
The error is probably that reads from a pair have equal names up to the first white space. You can add 1 to the first read and 2 to the...
Hmmm that is weird, I tried with both python2 and python3 and it works.