methylseq icon indicating copy to clipboard operation
methylseq copied to clipboard

Methylation (Bisulfite-Sequencing) analysis pipeline using Bismark or bwa-meth + MethylDackel

Results 80 methylseq issues
Sort by recently updated
recently updated
newest added

Hi all, Does this pipeline handles spike ins (like the PhiX from illumina)? thank you in advance

enhancement

Hi all, @FelixKrueger @ewels I tried to run the pipeline with some data, but I had no idea about how they have been produced. I know just that they are...

question

I noticed two hard-coded settings for bismark that are only for paired-end calls https://github.com/nf-core/methylseq/blob/62d30e1d06d194047a372be60e4755fd8d0e9281/main.nf#L511-L512 Should these be moved into parameters?

question

You may also want to look in to BitMapperBS, a new methylation calling tool, which seems very interesting given the Time and Sensitivity improvements over the other tools. Check the...

enhancement

Add the copyright holder to Licence file again.

Version `2.6` of [nf-core/tools](https://github.com/nf-core/tools) has just been released with updates to the nf-core template. This automated pull-request attempts to apply the relevant updates to this pipeline. Please make sure to...

- [x] Merge [#242](https://github.com/nf-core/methylseq/pull/242) to get up to date with the template - [x] Try to resolve nf-core lint stuff, eg. updating modules - [ ] Finish multicore Bismark module...

enhancement

### Description of the bug I have previously processed the GRCh38 genome with bwameth for a different pipeline that I'm trying to compare with the nf-core. So I provide the...

bug

### Description of the bug Hi. I'm encountering an error with the methylsig pipeline when using bwameth aligner. The pipeline runs fine with Bismark, but my mapping is low (~40%)...

bug