migec
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mikessh
A RepSeq processing swiss-knife
Hi! I closed the [issue](https://github.com/mikessh/migec/issues/53#issue-502801066) I'd opened recently since I realized it was a problem upstream in the process, up to the demultiplexing process (Checkout). As part of my *barcodes.txt*...
The descriptions for "--log-sample-name" and "--log-sample-type" imply that they would take string argument. However passing any arguments to either option throws a Bad file extension Error as Assemble interprets the...
I am currently working with a 400+200 MiSeq preped exactly as the MiGeC Nature Methods paper. However, during the initial checkout when I run the following code on my Linux...
Hi there, I like your software and want to use it for my samples. But they each have a different umi length. Could you please advise? Thank you.
if yes, this consider as master barcode or slave barcode? Thanks!
I was try test data of MISEG. The barcode.txt records the adaptor sequence + UMI (marked as N). The adapter sequences is either lower or upper cased indicating fuzzy or...
Dear all, I am new to this package. I have a somewhat non standard set-up with 3 fastq files : read 1, read 2 and index file. The index.fastq contains...
Hi, I suspect I have a problem I cannot really solve myself. I noticed that one gene we are looking for is actually just not there (IGHV1-9). Using MIXCR on...
Hi, Great tool! We have Single Cell TCR, and BCR reads sequenced from sorted cells and we use a set of in-house barcodes to identify the wells. We don't have...
