Marius Pachitariu

That's an interesting idea, I think we can do that. Would it be enough if only one ROI was active at any one time?

Would it be more useful to segment the whole image first, and then have a polygon ROI selection tool for counting? How large are the images that need to be...

Given the very large image sizes, do you think this should be done manually or offline as part of a batch processing pipeline? Optimizing the GUI for such large images...

Thanks for the idea for how to calibrate the cell diameter, we'll do that. Sounds like you are interested in some kind of image analytics downstream of segmentation. The quickest...

@ldavr I think that works as intended. The dat path should be set to the drift corrected file, otherwise you'll see the raw spikes moving with drift. The 383 channels...

So to replicate this problem, I should just set connected(n) = 0 for some channel n on NP 1.0 ?

Hi Virginia, we are still working on the paper, but we'll send you our CoSyNe abstract that has a little more information. - Marius

It is likely due to template subtraction. If it was the axon it would always go off together with soma.

Nick meant that you're over sampling relative to the duration of the spike. The easiest thing might be to bin your data so that trough to peak is about 10...

Sorry no, that was not a goal of this release, nor is the original strategy compatible with Kilosort4 (we don't have the non parametric tracking from Kilosort2). You can look...