LinXing Chen

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Hi, Thank you so much for your efforts. 1. regarding the header format from different mapping tools, have you tested if pysam could distinguish the "/1" and "/2" and remove...

Hi, Thank you for your interest in COBRA. You are right that, as you only had 9 queries and none of them was extended, thus the blast db is empty....

You could bin the (viral) contigs from a single assembly (not the assemblies from two or more samples), this is also true for COBRA, which can not deal with contigs...

unfortunately I did not test them, but I do not think they will perform very well, given the genomic features of viruses.

Good to know. thank you.

> Unfortunately, after updating to the latest version, the -q sequence is too small is reported as an error `no query was extended, exit! this is normal if you only...

> When the number of -q is sufficient, some sequences can be reported to be lengthened or even looped that's right

It is totally up to you. Some people reported low-quality genomes in their papers, you could do that as well.

sounds good.

> maybe this error happen with that the coverage.txt is empty。I meet the same error you will meet other type of error(s) if the coverage.txt file is empty i believe.