cobra
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Questions on input fasta files
Thanks for this great tool! However, I have a few questions on input for cobra
- Now I'm using megahit to assembly (I just know from #3 that it may generate many chimeric contigs, but spades may run out of memory when assembly environmental samples). In megahit intermediate results, it will provide a file named
./intermediate_contigs/k141.contigs.fa
, in which there are many small contigs < 200 bp (which will be filtered in./final.contigs.fa
). My question is, which contig file is more recommended to be used as--fasta FASTA
input? - Is it possible to just use the
final.contigs.fa
as--query QUERY
file, or a filtered version with all contigs longer than given length (i.e. 1000 or 2500 bp)? In another words, can cobra be used before virus contigs annotation and MAG binning?
Regards, hwrn