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A versatile pairwise aligner for genomic and spliced nucleotide sequences

Results 185 minimap2 issues
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Hi, big Peak memory of minimap2 in some case when WGS mapping . For some 1x WGS, the peak memory usage is about 15G, but for some 30x WGS, the...

Hello! When it comes to structural variant calling, one characteristic of the presence thereof is a noisier sequence than usual. With these noisy sequences, the secondary alignment can then provide...

feature-request

the manual says this about `-C`: `Cost for a non-canonical GT-AG splicing (effective with --splice) [0]` but what is a "non-canonical GT-AG splicing"? Is it meant to say `non-canonical (non-GT-AG)...

Hi, I have assembled a genome using nanopore long reads. No reference is available for this genome. In addition to BUSCO, I want to assess the completeness of this assembly...

Reproductive command steps for arm64 minimap2 using SIMDe, as asked in: https://github.com/lh3/minimap2/pull/597 In process, I have fixed an error in Makefile.simde that probably appeared during the merge of previous PR.

So for some reason, 2 out of many many many fastq are coming up with this error. I am not very bioinformatics savy. So may need some help solving this...

Hi Thanks for this great bit of software. I know it sounds like a funny request but I would like to compile minimap2 on our power9 GPU nodes - essentially...

Hi @lh3, My student Mohsen and I are using minimap2's ksw2 library in a few projects, and we noticed some behavior that I think is unexpected/undefined. He posted an issue...

For example, it doesn't report an error for preset "splice:hd".

bug

Hello, I am new to de novo assembly. I use minimap2, miniasm, racon and pilon to assembly genome(about 30M) but in the finally file I can't find the sequence of...