Edward Wallace

There is a lot of old RNA extracted from Heat-shocked yeast (by me in 2018) in the -80 freezer. If that RNA is still ok, then we could use it....

Trial functions added in commit 0c2e3f6. `make_row_names_LC_1536()` and `make_row_names_Echo_1536()` for row names in 2 different formats, `create_blank_plate_1536well()` as default call.

I *think* these are fixed. We need to test them as we set up the experiments with @j-aux, and then hopefully close the issue.

Created new branch `setup-1536well-5` with some updates in commit 39efde9 * `read_lc1536_1colour_cq` function reads in lightcycler 1536-well plate data * `read_lightcycler_1colour_cq` and `read_lightcycler_1colour_cq` explain that they work for lightcycler 480...

@DimmestP please update this ticket if you have any info.

I just looked at this. The code supports it by editing `dilution` and `dilution_nice` arguments. I think we can address this by explaining in the documentation string, and it won't...

We said in our resubmission that we decided adding this vignette was not essential and so prioritised other things. Then @jooolia's review did not ask for it. So, this is...

Also see [System-specific periodicity in quantitative real-time polymerase chain reaction data questions threshold-based quantitation](https://www.nature.com/articles/srep38951), which reviews existing methods and recommends "workers should either use scale-insensitive Cqs or normalize their growth...

Discussed 4 September 2020: we are prioritising features *downstream* of Cq calculation for now, looking towards rOpensci submission. We might make Cq calculation a higher priority later, for example if...

Our updated vignettes do a better job of explaining `sample_id` as the unique sample identifier. However we're not sure whether they do a good enough job introducing the split/apply/combine data...