Edward Wallace
Edward Wallace
I will draft and sent to @DimmestP today.
_Draft email here - edited in response to Aidan's comments_ Subject: qPCR analysis open-source R package Dear Colleagues, Do you use quantitative PCR in your work? Would you like to...
Better recommendation: use [plater package](https://github.com/ropensci/plater) to include data "laid out like plates".
@seaaan, this is a feature that we would be happy to add with a new vignette, do you by any chance have some qPCR date with a plater setup that...
Thanks @seaaan , the attachment didn't come through though. Any chance you could add the data and plate plan to directory `/inst/extdata`, then pull request into `plater-excel-12` branch?
This would be really nice as a separate vignette but is low-priority for now. We will revisit when we come to make teaching materials, as alternate graphs would be very...
Agree this would be desirable, we will discuss informally and come up with some ideas.
Discussed as a hint in platesetup_vignette.Rmd, as updated in #37.
We are waiting to address this until a user asks for it and provides some example plate layouts and data. Might be addressable with plater, related to #12.
See also [relative quantification framework paper](https://genomebiology.biomedcentral.com/articles/10.1186/gb-2007-8-2-r19), including taking a mean of efficiencies for normalizing acorss multiple reference genes. That 2007 paper refers to qbase as an open-source software, but I...