scasa

scasa copied to clipboard

fail in docker: Total 0 white-listed Barcodes

9

Thanks for your work. Everything works well with the demo testing data. However, when I have tested scRNAseq data from 10xgenomics 3' v2. Although the first part runs well. all...

LinAU

Using your tool for single cell data barcoded with split-seq technology and not droplet based

1

Hello, thank you for the very interesting tool. I am having single cell data barcoded with the split-seq technology and I would like to do a prediction with the shorts...

vasikara17

So many Error messages: please help

13

Directory ./ already exists. Writing into existing directory.. mkdir: cannot create directory ‘.//SCASA_testscasaHNVC02_20230414001259/’: File exists Preparing for alignment.. Indexing reference.. Directory .//SCASA_testscasaHNVC02_20230414001259/0PRESETS//REF_INDEX/ already exists. Writing into existing directory.. Version Info:...

RenaeAtkinson

Smart-seq2 full length RNA-seq data

1

Dear Author, Thank you for the wonderful tool. I wonder if it can analysis Smart-seq2 full length single cell RNA-seq data as well. If it can, what should be come...

seongguheo

Alevin Failed

10

When I used the example data to test the program, I get an error ```bash Error in file(con, "r") : cannot open the connection Calls: readLines -> file In addition:...

SoberDog

Scasa:v1.0.1 docker shows error

3

I am trying to use the scasav1.0.1 docker in a Mac M1 system and I get the following error when running the test dataset. The main issue is that it...

morgan-th

Quantification Fails

3

Hi, Thank you for the tool. I am using it for isoform quantification. As a test run I am testing it on one sample from 10xV2. The command that I...

abhideeplife

Running scasa from a symlink is not possible as Scasa expects to be able to access the scasa directory via the "which scasa" command (see SCASA_WRAP_V1.0.0.pl line 36), however, no...

MehtaKnight01

Hi, In the PERL script "SCASA_WRAP_V1.0.0.pl", 'die' commands that specify a color permanently change the color of the command line interface after the scasa program exits. Please see https://stackoverflow.com/questions/5691570/coloring-a-perl-die-message for...

MehtaKnight01