hifiasm
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chhylp123
Hifiasm: a haplotype-resolved assembler for accurate Hifi reads
Hi, Thanks for your nice hifiasm. I was using hifiasm to assemble triploid recently, and i used --n-hap 3, but i just got *hap1* and *hap2* without *hap3*. It seems...
Hello 👋 , We've encountered multiple instances with our non-trio male assemblies, where both haplotypes contain contigs that map to chrX. Here's an example with HG00731 (pacbio ccs reads, 33X...
Hi! I'm trying to reduce the number of contigs in an assembly I have that was initially put together in SMRT analysis software but getting a much shorter assembly than...
Hi, Thank you for making this excellent tool. I was wondering whether I should set up `--n-hap` to 10 for my hifi reads comprising 5 individual of small insect individuals...
Dear authors, We have a genome about 5G, and the amount of HIFI data is about 134G. We want to get a better preliminary assembly result with hifiasm software with...
Hi, I ran Hifiasm on Hifi reads derived from an inbred strain of XX/XY species. In order to determine Y chromosome sequences, I extracted genomic DNA from male. The result...
Hi, I am assembling a small genome (< 500Mb) with aprox. 180x Hifi read data. Here is the command used: `hifiasm -o results -t 60 -l0 reads.fastq.gz` I am running...
Dear authors, I am applying Hifiasm to assembly our Hifi read data (DNA from a single individual for each species). However, we notice that the duplication rate (based on BUSCO)...
Hi,I want to know the reason for the low N50.Is one of this: 1、low read depth, and what is the minimum read depth to extend one contig? 2、if a het...
I am using PacBio Hifi yeast genomes for this assembly and I would like to understand what the data is telling me: if the sequencing job was bad, if my...
