FastQ-Screen
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Detecting contamination in NGS data and multi-species analysis
Hello, it is possible to use pigz for parallel gzip operations? I've allowed 10 threads for fastq_screen, but only a single thread is used for gzip. It would be super...
Devise a way to handle very long reads (e.g. Oxford Nanopore). Maybe make compatible with Minimap2 - as discussed by email with users. Alternatively, break up reads and process with...
Create a rRNA genome for download (--get_genomes)that comprises multiple species and document this in the config file.
The message below could be more elegant: (base) ultraviolet@ultraviolet-X555QA:~$ fastq_screen_v0.13.0/fastq_screen fsq_test_dataset.fastq.gz Using fastq_screen v0.13.0 Reading configuration from '/home/ultraviolet/fastq_screen_v0.13.0/fastq_screen.conf' Aligner (--aligner) not specified, but Bowtie2 path and index files found: mapping...
Make FastQ Screen available on DockerHub
fastq_screen does not yield non-zero exit code when aligner returns non-zero exit code. This seems like a problem since the expected output files are all made, but the reported values...
Running ```shell fastq_screen --get_genomes ``` as instructed [here](https://stevenwingett.github.io/FastQ-Screen/#obtaining-reference-genomes) will return a `404 NOT FOUND` against https://ftp1.babraham.ac.uk/ftpusr46/FastQ_Screen_Genomes/
If you have an RNA sequence with U's, the U's will have to be converted to T's to get an alignment. Just something users might want to be aware of.
Add minimap2 as an aligner option to support long reads, etc.