Shifu Chen

> @angusdavison Are you sure you're not seeing a dependency conflict? Fastq requires a newer version of libdeflate, which means that if you have e.g. BWA in your environment, you...

But fastp dons't declare a dependency on libstdcxx-ng[version='>=9.4.0']. I checked annocanda, the latest version for libstdcxx-ng is 9.3.0.

Thanks for your info. Which version did you use? Can you upload a file with the first 100K reads here, so I can reproduce the problem? You can remove `--detect_adapter_for_pe`...

Thanks, I will find a chance to reproduce this issue.

Will consider to implement this feature. Could you guys let me know what's the NNNNNNNNNN designed for ? UMI or barcodes in single-cell sequencing?

Since using >16 threads cannot make fastp faster. The bottleneck is I/O when thread number > 8.

Could you please give me some examples of the `patterns`?

And, is regex required, or is it enough to support just N base?

1, duplication evaluation for SE data is always inaccurate, since you never know where is the another end of the DNA fragment. For RNA data, it is more serious. 2,...

But fastp uses the original data to calculate duplication level.