Chang Ye

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Is it necessary to add job_exec_prefix for remote executor? Similar to the local executor: https://github.com/snakemake/snakemake/blob/6a0b5211d6c2c92ff37c939bdeb4ee2b4c0e3572/snakemake/executors/local.py#L79-L80

Thank you @endrebak for the information about version 1.x.

close https://github.com/marcelm/cutadapt/issues/804

Hi @haowBio, Sure. I have another repo called metagene, which can annotate genome coordination into gene coordination. I will add more instruction in the document and keep you posted.

Hi @cavelandiah, Thank you for using this pipeline. The reply to your questions is as below. - Does pseudo-BID-seq apply the same analysis logic to the genome and genes references...

And it seem that gem3 failed to choose the best alignment as the primary alignment. As shown in this example output. Ref "U-25%-(V)" is be better than "U-0%-(V)" but this...

Testing data: [debug_markdup.zip](https://github.com/user-attachments/files/20151490/debug_markdup.zip)

Thank you for the reply. In the example above without taking secondary alignment into account, the non-duplication rate for primary alignment would also be overestimated

Thank you for you interest, deletion filter is a simple script, I will posted a new repo for this and let you know.