DiaNN issues

Parameters for DiaNN library free phospho search

2

Hello! I would like to run a library free search on some diaPASEF data of phospho enriched samples. Would you be able to suggest the optimal parameters for this? So...

aparnasrini03

Could anyone please share their MS DIA methods or settings here?

6

Hi, I am currently using Thermo Scientific Q Exactive mass spectrometer using ESI ion positive mode with a Phenomenex Kinetex 2.6 μm XB-C18 100 Å (150 × 4.6 mm) column....

chingciripit

Update README.md

1

Added some missing command line options.

npinter

WARNING: incorrectly recorded isolation window margins

2

Hi all, I have a issue with PASEF .d files that I have and I want to generate a library. As you can see in the image below the program...

luoxing1996

Different protein group for same peptide different charge state

3

I noted something strange today after I was asked by a colleague to add a number of peptides column to her DiaNN pg matrix. I loaded the report.tsv in R,...

Arthfael

Does --vis option work for timsTOF data?

3

The --vis works fine for Sciex and Thermo data. But when I use it with .d files it seems the XIC.tsv files are not generated. Is that the expected behavior...

volpato30

No data in matrix file despite of main report shown : DIA-nn

2

Hi DiaNN team, I ran DiaNN search using library from Fragpipe and diaPASEF (.d) were used as input. When, the programme run and got the results. Below is main report...

NarainritKaruna

Unknown modification in spectral library

1

Hello Vadim, I tried to load a spectral library from this repository: https://github.com/ZiluYe/Glyco-DIA These are generated spectral libraries with glyco modifications from Spectronaut. When I try to search against this...

MarST07