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                                            Sharpy

The bam file does not contain cell and umi barcodes appropriately formatted.(use SRP129388 human week 10 fetal forebrain dataset in your paper )

@songxiaoning can you please tell you machine configurations, as i also have 20gb bam file like yours, and my velocyto run command gets an error message killed while creating loom...

How to create seurat object from a loom file directly??

This tutorial does not show how to create seurat object from loom object. Can you please elaborate on this?