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AMPtk: Amplicon ToolKit for NGS data (formally UFITS)

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I was learning amptk, and ran AMPtk Quick Start guidling to process test data from and I got this error when filtering. ################ Traceback (most recent call last): File "/home/user1/miniconda3/envs/amptk154/bin/amptk",...

Hello, I am using amptk to analyze some paired-end COI diet data from bat feces. I have mostly gotten the pipeline to work, except for the amptk -filter step. My...

When I tried to build custom COI database,I got this error in step #generate UTAX database ##########this is my code amptk database -i arth10000.BIN-consensus.fasta -f LCO1490 -r GGWACTAATCAATTTCCAAATCC --primer_required rev...

Getting this error ``` Traceback (most recent call last): File "/opt/linux/rocky/8.x/x86_64/pkgs/amptk/1.6/bin/amptk", line 8, in sys.exit(main()) File "/bigdata/operations/pkgadmin/opt/linux/centos/8.x/x86_64/pkgs/amptk/1.6/lib/python3.10/site-packages/amptk/amptk.py", line 784, in main mod.main(arguments) File "/bigdata/operations/pkgadmin/opt/linux/centos/8.x/x86_64/pkgs/amptk/1.6/lib/python3.10/site-packages/amptk/OTU_cluster.py", line 190, in main numKept, numDropped...

Hi, I'm currently unable to successfully install AMPtk. Following the installation instructions on https://amptk.readthedocs.io/en/latest/ - I type in the first three commands: conda config --add channels defaults conda config --add...

problem is reformatting the taxonomy information in format needed by UTAX/SINTAX. I've previously looked at SILVA taxonomy -- the taxonomy appeared to be a hot mess (I'm not a bacteriologist).......

enhancement

I am trying to cluster my data using unoise3 with the following command: amptk unoise3 -i out_lr22.demux.fq.gz -o out_lr22 -p 98 -e 2.0 --usearch usearch10 I know usearch is no...

I have some sets of dual indexed fungal ITS amplicon pools from specimens. One is 288 specimens and the other is 480 specimens if they would be helpful at all...

Hi there, I am new to this package, when I try to install amptk from conda, I had no luck.. could anyone help me? Thanks in advance. First, I created...

Cross reference #87, ``amptk_mock1.fa`` was earlier work and does not have species names. Pulling out the species names, ``` $ grep "^>" amptk_mock[2-3].fa | cut -f 2 -d ">" |...