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Tombo resquiggle error

5

Hi, After basecalling with guppy, I using tombo to resquiggle the fast5 files of nanopore direct RNA-seq data. However, I checked the log and got the result as follows: **_Final...

q1134269149

Tombo resquiggle error

1

Hi, I am trying to resquiggle some fast5s but am running into the following error: ``` Traceback (most recent call last): File "/opt/conda/bin/tombo", line 8, in sys.exit(main()) File "/opt/conda/lib/python3.9/site-packages/tombo/__main__.py", line...

rowanhowellGE

[resquiggle] All my reads were unsuccessful

1

Hello, marcus1487 This is my first methylation analysis with tombo using nanopore sequencing data. I hope you understand, even if this is a rather vague question. I used the 'tombo...

JAEYOONSUNG

tombo resquiggle for r10 data

1

Hi, Thank you very much for this very useful tool. I am very interested in this tool. I just would like to know whether tombo can be used for nanopore...

Flower9618

Hi Tombo Team! I want to know the exact meaning of fit-global-scale parameter when using Tombo resquiggle？and what it is used for? and when I should use ? I used...

xieyy46

There are several typos here that I fixed.

rseymour1

Hi Marcus, I'm hoping to analyze m6A modification for a set of direct RNA data from ONT. Right now I can only use de novo detection on tombo, and I'm...

sidizhao

Hi, I try to plot genome_location function using command: ``` tombo plot genome_locations --fast5-basedirs fast5_dir \ --genome-locations chr1:17114185 --num-bases 100 \ --pdf-filename plot.r100.pdf ``` then get an error: ``` res...

yipukangda

Readonly mode when preprocessing fast5

8

Dear developers, When trying to add fastq to fast5 files with `tombo preprocess annotate_raw_with_fastqs` I found that fast5 files are opened in readonly mode and fastq strings cannot be added....

DNKonanov

Hello, I am running this tombo command with etect_modifications [00:54:29] Loading minimap2 reference. [00:54:47] Getting file list. [00:55:52] Loading default canonical ***** DNA ***** model. [00:55:58] Re-squiggling reads (raw signal...

HaoWangLYL