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A fast and robust pre-processing pipeline for bulk or single-cell whole-genome bisulfite sequencing (WGBS) data.

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Hi, There seems to be a problem with the docker image, I am unable to download MethylStar: > wget www.jlabdata.org/methylstar.tar.gz --2022-08-31 14:09:11-- http://www.jlabdata.org/methylstar.tar.gz Resolving www.jlabdata.org (www.jlabdata.org)... 129.187.153.217 Connecting to www.jlabdata.org...

Hello, after running deduplicate, I did run Genome coverage & Sequencing depth. I got the warning: WARNING: Genome (-g) files are ignored when BAM input is provided. Now I run...

Hello, I have properly formatted all of the parameters necessary for running the program (see below), but somehow am still getting the error message: ./src/bash/preparing.sh: 5: ./src/bash/tmp.conf: genome/: not found...

Hi team, I have set up methystar on my system. Thanks for the simple and seamless process. While running the pipeline, there's an issue in the Bismark mapping step. The...

Dear madam/sir, Currently, I am setting up all the pre-processing steps for analyzing my cell-free DNA sequencing data, expected to arrive any time soon. While exploring all the possibilities for...