Alec hong

when build the dockerfile, it get error lying below: [root@iZ0xi7sue1xrh5f0xxhrdiZ popscle-master]# docker build . [+] Building 2.3s (9/14) docker:default => [internal] load build definition from Dockerfile 0.0s => => transferring...

The cpu resource exhausting

1

Hi, It no doubt that the cellassign was great tools for personalization cell annotating. on my case to running the data, something of, taking cellassign would be invoked so many...

Hi, I want to install the cellassign R package using miniconda that install with other path not the default miniconda path like /home/user1/miniconda3. For example, I install the miniconda on...

Had any scripts to pick the distinctive SNP site from vireo file GT_donors.vireo.vcf.gz gene to distinguish multiple sample using PCR experiment

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Hi development, There are question about the genes selected to demultiplex the mixed samples using PCR experiment. the result files "GT_donors.vireo.vcf.gz" had been generated by vireo pipeline, however i want...

how can i build the preprocess vcf for mouse, like human common SNP vcf file, using GATK google warehouse snp data

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Hi development, I want to demultiplex the mouse single cells data, but the SNP file can not be obtain. so i want to build it myself from the GATK mouse...

I don't want to use the docker to run the task

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Hi developer, I don't want to use the docker to run the task, based on this, how to set the wdl file not to use the docker, i just want...

error in startup

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Hi, When i start up this app, it give me above error.   how can i fix this? any advice would be thanks. Best, hanhuihong

[BUG] the binary binary_mtx, auc_thresholds = binarize(auc_mtx, num_workers = 15) get error

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**Describe the bug** when i try to binarize the auc file in pyscenic final step, it getting error with : BLAS : Program is Terminated. Because you tried to allocate...

can i take the spatial spot as singlecell to run scRNA group cellchat analysis

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Hi developer, I had 8 10x spatial data, and i want to output the single cell(scRNA) plot, and then i want to runing the spatial spot like single cell, can...

why monocle2 display error on orderCells step?

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Hi @ctrapnell Thanks to developing the great tools for pseudotime analysis in singlecell. I use monocle to make a pseudotime anlysis with my single cell sequencing data. After dimension reduction...