Ben Parks
Ben Parks
Hi @Dario-Rocha, How much memory did you have allocated on your server when you were running `install_github`? The error `x86_64-conda-linux-gnu-c++: fatal error: Killed signal terminated program cc1plus compilation terminated` suggests...
Hi @harper500, we don't build our own conda packages, but there are some 3rd party efforts to host BPCells as a conda package. There's a PR to add BPCells v0.3.0...
Hi @Feilijiang, this is a good question! Many batch-correction methods such as Harmony operate on the PCA matrix, not the full RNA counts matrix. Disk-backed calculations with BPCells can be...
Ah, amusingly this is an error in my error message and it will be fixed with the pull request linked above. Ultimately, though, your problem is that the matrix you...
Hi @Flu09, either approach should be fine and all downstream operations should work regardless of the choice you make. `saveRDS()` is probably the easiest approach, just remember that the BPCells...
This error looks like it's more on the Seurat side. From the error message, I think that Seurat must be calling a function that expects to receive a `DelayedArray` object...
From your example, I think a problem you have is that you have already normalized your Seurat object prior to moving the BPCells folder. Therefore, the data layer (`obj2[["RNA"]]$data`) will...
Hi @Liripo, thanks for your question! Overall, yes this is something that would be possible. First, I think it might be better for me to understand what you want to...
Ah, if you're interested in AUC calculations I think that can be accomplished with just a slight modification of the existing wilcoxon code in `marker_features()`. Looking this up on [wikipedia](https://en.wikipedia.org/wiki/Mann%E2%80%93Whitney_U_test#Area-under-curve_(AUC)_statistic_for_ROC_curves),...
Hi @Liripo, sorry for the delay on this. I've just added a pair of functions that I think will help you a lot if you want to implement something like...