Bo Li

@mourisl @haowenz , does it make sense to add another option (e.g. '--barcode-reversion') to reverse complement barcode FASTQ? As @dannyconrad mentioned, Illumina's new machines (e.g. NextSeq, NovaSeq) implement the reverse...

@mourisl, awesome, thanks a lot for keep developing this great software!

@mourisl , I detected one bug related to this new feature. When I set '--read-format bc:0:-1:-', I still got the following error message: Less than 5% barcodes can be found...

Hi @mourisl , yes, it works. However, we are building workflows, which means we do not have control of what white list people will provide (either gzipped or not). We...

@sekhwal , do you want to install RSEM for nanopore data?

https://deweylab.github.io/RSEM/README.html#compilation

https://anaconda.org/bioconda/rsem

@sekhwal , unfortunately, RSEM does not handle MinION data right now.