Benjamin Buchfink
Benjamin Buchfink
The problem is probably the long input sequences which is not efficient in regular blastx mode, try setting `-F 15`. Additionally I'd recommend `-b4 -c1`.
You seem to be using v0.9.14 for which you might need to input that file gzip compressed. I would recommend though updating to the latest version which does not need...
N/A means that the target sequence does not have any taxids assigned to it. 0 can mean that the target's taxon id is not mapped to a taxonomic rank (possibly...
If you want to run diamond blastx on raw contigs without gene calling, you should use the frameshift mode (`-F 15`). It is normal that the output file remains empty...
This happens due to masking of frequent seeds. You can find the hits in ultra-sensitive mode by setting `--freq-sd 50`. It may be worth tuning some parameters here if you...
> > > Ok but I don't understand why in more-sensitive mode using `--freq-sd 20` (the default value of ultra-sensitive) I found the same hits than with `--freq-sd 200` (the...
This does not look like a disk space problem to me. Please try using the option `--no-unlink` and see if that helps.
Not sure which version it was added, but it is in the latest version yes.
Have you compiled the latest master branch by any chance? If so, you need to add the CMake option `-DEXTRA=ON` to activate this option.
It prevents unlink being invoked on the temp files, which normally will cause these files to be deleted even if the program crashes etc.