running time estimation

[distilledchild]

Hi, I am running blastx with rat genome assembly. Even though I am using multi core and enough ram, how much time it will take? It seems it is running, but still output is 0 records. Is there any way to make this work faster ?

Thank you.

diamond blastx
-q /rat.fasta
-d /uniprot_2021_06/reference_proteomes.dmnd
-f 6 staxids bitscore qseqid sseqid pident length mismatch gapopen qstart qend sstart
--log -v
--sensitive --max-target-seqs 1 --evalue 1e-25 -p $thread --masking 0 -b 0.2
-o diamond.out

[bbuchfink]

If you want to run diamond blastx on raw contigs without gene calling, you should use the frameshift mode (-F 15). It is normal that the output file remains empty for a long time, since output is first written to temp files.