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An error message in stringtie's mix option

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Hello, When I use the -mix parameter, I consistently encounter an error message that says "Segmentation fault". Can you give me some advice? the parameters are the following: stringtie -o...

Omissions due to sample name

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### Description of bug Hi, when I use the multiqc to summarize the results of qc. I noticed that there some results are omitted due to the word "of" in...

Hi, I'm going to assemble transcripts by each tissue. Firstly, I'll merge all sorted bam files form the same tissue. and then use the merged bam file to get the...

Hello, I'm using stringtie:2.2.2 to assemble transcripts by --mix model. All samples were finished successfully except one sample. and I separated the bam file by chromosome to check the bam...

Hi, [bulk mode] salmon 1.10.3 the error was reported in partial jobs when I used salmon to quantify the LR-RNA-seq data (It works normally on some samples). `singularity exec /public/home/b20223040336/Workspace/long_read_rna/02code/singularity_images/salmon:1.10.3--h6dccd9a_2...

Hi, I hope you're well. Here is my question: [Bulk mode] Error: Transcript * appears in the reference but did not appear in the BAM I want to obtain the...

About multi-tissue assembly

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Hi, I want to assemble a final set of transcripts from multiple tissues, But I have a question. Should I first assemble transcripts for each sample, then merge the transcripts...

Hi, When I assemble transcripts using StringTie2 (-mix), the process is terminated by signal 11. I noticed that more than 70% of the reads are aligned to chromosome 6 (in...

Question about long processing time for specific conditions in DAS analysis

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Hi @EduEyras @leipzig , I'm currently running DAS across 100 conditions with the following code: `sbatch -p Cnode_all -c 3 --mem=60GB -J SR_fix_transcript_SE_DAS --time=30-00:00:00 --wrap='singularity exec /public/home/b20223040336/Workspace/long_read_rna/02code/singularity_images/suppa:2.3--py36_0 suppa.py diffSplice --method...