Tang JIWEI

Hi, @andredsim Yes, I heard it's great in quantitative terms, Thank you!

Hello, @apcamargo Here I got the protein sequence obtained by Rnasamba, but there is no cds sequence. I used TransDecoder to predict the ORF, and used the protein sequence obtained...

Hi, @apcamargo Excuse me again Through this method, the original protein sequences predicted by RNAsamba were 174,084, but the cds sequences obtained through TransDecoder were only 171,513. How should we...

Hi, @apcamargo I got it, Thank you!

@ArtRand [Y5dm.log](https://github.com/user-attachments/files/20104529/Y5dm.log) previous [Y5dm_err.log](https://github.com/user-attachments/files/20104538/Y5dm_err.log) After updating to the new version, I also included the --careful parameter

@ArtRand Thanks for your reply! > One thing, I'm not sure if this applies to your analysis however, is that the [MAP-based p-value](https://nanoporetech.github.io/modkit/dmr_scoring_details.html#map-based-p-value) metric in modkit dmr is a "modified/un-modified"...

@ArtRand > My guess is that when you use --ignore 17596 that some of the m6A calls fail the pass threshold. The threshold is estimated on the adjusted probabilities and...

@alephreish Okay, thanks.

 Hi @andrewprzh The top GTF is the original reference GTF obtained from short-read RNA-seq (containing only the gene level as a reference), the second is the transcript_models.gtf obtained by...

@andrewprzh Thanks! The data involves confidentiality, so I've sent it directly to your email.