Tang JIWEI
Tang JIWEI
Primary assembly. Furthermore, karyotype analysis was performed, confirming it is a diploid organism
@Psy-Fer I understand, What software to use to split POD5? And The strange thing is that one of the samples also had an error, but it ran successfully. POD5(532G) to...
@Psy-Fer First of all, I'm sorry to disturb your vacation. Where do I get the Acquisition ID? This split is not an easy work.
@Psy-Fer First of all, thank you very much for your patient reply. I personally think this function is very important. I hope you can implement this function when you are...
@hasindu2008 In November, I sent my plant samples for sequencing to a company. The company returned POD5 files to me. I sequenced 12 samples, with an average of 700 GB...
@hasindu2008 okay， I will jump in here.
@Psy-Fer My data is RNA 004, and I am new to the DRS project, aiming to analyze m6A modifications and polyA tail length. Initially, I planned to follow the traditional...
@hasindu2008 Another question. Among my 12 samples, fortunately, a few were successfully converted to BLOW5. However, when I ran this command: `f5c eventalign --rna -r Y1-10-2/pass.fq.gz -b Y1_10_2.bam --genome Y1.fa...
@hasindu2008 Okay, as a beginner, I didn't expect it to be this large. I'm using CPU with --threads 20. How long would this take?
@hasindu2008 As shown below `lscpu` Architecture: x86_64 CPU op-mode(s): 32-bit, 64-bit Byte Order: Little Endian CPU(s): 12