Tang JIWEI
Tang JIWEI
@sanyalab Hi， If you just wants to construct a relatively complete full-length transcriptome, I think it is possible to do so.
@ArtRand Thank you! The dorado step was performed by a partner company. However, I still have a question. I have two species, which are allotetraploids belonging to the same genus....
@ArtRand Thank you for your patient reply! I have another question: The pass.fq.gz file provided by the sequencing company already contains methylation modification information and polyA tail length. Can I...
Hi @ArtRand the BAM file generated from aligning sequencing reads to a transcriptome I have a doubt regarding the following commands: Without using --ref: `modkit pileup Y2_5_2.bam Y2_5_2.bed --log-filepath Y2_5_2.log...
@ArtRand Regarding the filtering of positions based on `percent_modified`, I have set a threshold of >20%. Is there any published or commonly accepted justification for using this threshold?  Secondly,...
@hasindu2008 hi, so Can f5c also calculate polyA length?
@hasindu2008 Okay ,thank you! If it is RNA004, what methods can be used to calculate the polyA length?
@hasindu2008 Yes, But this step is coducted by sequecing company, because I don't have GPU. Okay, thank you again!
Sorry, It's my carelessness
@XqKang If the data you are using is ONT RNA004, I suggest you use dorado+modkit, nanopolish does not support POD5, you need to convert POD5 to BLOW5